Sunday, 22 November 2015

Downstream Processing Overview

Todays Knowledge Information:
* Downstream processing *
Downstream processing refers to the recovery and purification of biosynthetic products, particularly pharmaceuticals, from natural sources such as animal or plant tissue or fermentation broth, including the recycling of salvageable components and the proper treatment and disposal of waste.
It is an essential step in the manufacture of pharmaceuticals such as antibiotics, hormones (e.g. insulin and human growth hormone), antibodies (e.g. infliximab and abciximab) and vaccines; antibodies and enzymes used in diagnostics; industrial enzymes.
4 Steps in Downsream Processing:
1. Removal of insolubles:
This is the first step and involves the capture of the product as a solute in a particulate-free liquid, for example the separation of cells, cell debris or other particulate matter from fermentation broth containing an antibiotic.
2.Product isolation:
This is the removal of those components whose properties vary markedly from that of the desired product.
3. Product purification:
This is done to separate those contaminants that resemble the product very closely in physical and chemical properties.
4. Product polishing:
This describes the final processing steps which end with packaging of the product in a form that is stable, easily transportable and convenient.

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